| 通过研究改性壳聚糖与细胞的相互作用评价其生物相容性 |
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| 引用本文： | 张淼,牟阳灵,公衍道,赵南明,张秀芳.通过研究改性壳聚糖与细胞的相互作用评价其生物相容性[J].生物物理学报,1999,15(4):640-646. |
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| 作者姓名： | 张淼 牟阳灵 公衍道 赵南明 张秀芳 |
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| 作者单位： | 清华大学生物科学与技术系生物膜与膜工程国家重点实验室!北京100084，清华大学生物科学与技术系生物膜与膜工程国家重点实验室!北京100084，清华大学生物科学与技术系生物膜与膜工程国家重点实验室!北京100084，清华大学生物科学与技术系生物膜与膜工程国家重点实验室!北京100084 |
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| 基金项目： | 国家863 计划基金!项目编号： 819 － 07 － 01 |
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| 摘 要： | 利用细胞生物学的方法， 研究了四种不同的细胞在经过改性的壳聚糖（ＣＨＩＴＯＳＡＮ） 膜上的生长，测定了细胞相对黏附力、细胞初始黏附率， 并利用ＦＤＡ 实验测定了细胞活力，从而从多个方面评价了这几种不同材料的生物相容性。实验结果表明，与明胶交联的壳聚糖膜明显比其它两种膜有利于细胞的黏附和生长，为进一步对材料进行筛选奠定了基础。
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| 关 键 词： | 改性壳聚糖 生物相容性 初始黏附率 细胞粘附力 FDA |
EVALUATION ON THE BIOCOMPATIBILITY OF MODIFIED CHITOSAN FILMS BY STUDYING THEIR INTERATION WITH CELLS |
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| Zhang Miao,Mu Yangling,Gong Yandao,Zhao Nanming,Zhang Xiufang.EVALUATION ON THE BIOCOMPATIBILITY OF MODIFIED CHITOSAN FILMS BY STUDYING THEIR INTERATION WITH CELLS[J].Acta Biophysica Sinica,1999,15(4):640-646. |
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| Authors: | Zhang Miao Mu Yangling Gong Yandao Zhao Nanming Zhang Xiufang |
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| Abstract: | In this study, the biological attributions of three different chitosan films were evaluated and compared. Four kinds of cells were cultured on modified chitosan films and their morphologies were observed. The initial anchoring rate and adhesion force were measured to evaluate the difference on the affinity of cells with chitosan films. FDA assay showed all these materials have no cell cytoxicity. The results of these experiments indicate that crosslinking of chitosan with gelatin can improve the affinity of materials with cells and is in favor of cell growth. |
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| Keywords: | Modified chitosan Biocompatibility Initial anchoring rate Adhesion force FDA |
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