Proteomics without polyacrylamide: qualitative and quantitative uses of tandem mass spectrometry in proteome analysis |
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Authors: | Goodlett David R Yi Eugene C |
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Institution: | (1) The Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103-8904, USA, |
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Abstract: | Proteomics can be thought of as an attempt to understand the information encoded in genomic sequences from the perspective
of proteins; i.e. the structure, function and regulation of biological processes at the protein level. In practice it stands
in stark contrast to the hypothesis-driven serial approach practiced in the last century that was so successful for protein
chemists and is built on the basic understanding of protein physicochemical properties developed during that era. Proteomics
attempts to study biological processes comprehensively or globally by systematic parallel analysis of proteins expressed in
a cell. While there are many analytical techniques in use and under development in proteomics, mass spectrometry is currently
one of the field's most important discovery-based tools. This article will review some of the current approaches for qualitative
and quantitative uses of tandem mass spectrometry in the field of proteomics specifically avoiding a discussion of the use
of gel electrophoresis prior to mass spectrometry.
Electronic Publication |
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Keywords: | Proteomics Tandem mass spectrometry Multi-dimensional chromatography Quantification |
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