The sugarcane bacilliform badnavirus promoter is active in both monocots and dicots |
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| Authors: | Tzafrir Iris Torbert Kimberly A. Lockhart Benham E. L. Somers David A. Olszewski Neil E. |
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| Affiliation: | (1) Department of Plant Biology and Plant Molecular Genetics Institute, University of Minnesota, 220 Biological Sciences Center, 1445 Gortner Ave., St. Paul, MN 55108, USA;(2) Present address: Plant Molecular Genetics Institute, University of Minnesota, 220 Biological Sciences Center, 1445 Gortner Ave., St. Paul, MN 55108, USA;(3) Present address: 1445 Gortner Ave., St. Paul, MN 55108, USA;(4) Department of Plant Pathology, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN, 55108, USA. |
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| Abstract: | Regions of the sugarcane bacilliform badnavirus genome were tested for promoter activity. The genomic region spanning nucleotides 5999–7420 was shown to possess promoter activity as exemplified by its ability to drive the expression of the coding region of the uidA gene of Escherichia coli, in both Avena sativa and Arabidopsis thaliana. In A. sativa, the promoter was active in all organs examined and, with the exception of the anthers where the expression was localized, this activity was constitutive. In A. thaliana, the promoter activity was constitutive in the rosette leaf, stem, stamen, and root and limited primarily to vascular tissue in the sepal and the silique. The transgene was inherited and active in progeny plants of both A. sativa and A. thaliana. |
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| Keywords: | Arabidopsis thaliana Avena sativa badnavirus promoter constitutive and vascular expression GUS staining transformation |
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