Isolation of an antigen fromBlastomyces dermatitidis that is specific for the diagnosis of blastomycosis |
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| Authors: | James H Green W Knox Harrell James E Johnson Robert Benson |
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| Institution: | (1) Bacterial and Fungal Products Branch, Biological Products Division, Center for Disease Control, Public Health Service, U. S. Department of Health and Human Services, 30333 Georgia, Atlanta, USA |
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| Abstract: | The A antigen ofBlastomyces dermatitidis has been isolated and purified by DEAE column chromatography. In the complement-fixation test, the antigen reacted with 10
of 16 sera from patients with proven cases of blastomycosis and was negative with known positive sera from 7 cases of histoplasmosis,
5 cases of coccidioidomycosis, 5 cases of candidiasis, and 5 cases of cryptococcosis. In the enzyme-immunoassay test, 25 of
27 sera from cases of blastomycosis were positive, but all heterologous and normal sera tested were negative. The antigen
gave a positive skin test with guinea pigs sensitized with killed yeast-phase cells ofB. dermatitidis and negative skin tests with guinea pigs sensitized with killed yeast-phase cells ofHistoplasma capsulatum. |
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