Nucleolar localization of ‘template-bound’ RNA polymerase I in nuclei of Xenopus laevis blastulae |
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| Authors: | Monique Boloukh re, Christian Thomas, Viviane Heilporn-Pohl, Fran oise Hanocq,J. Brachet |
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| Affiliation: | Laboratory of Molecular Cytology and Embryology, Department of Molecular Biology, Free University of Brussels, 1640 Rhode-St-Genèse, Belgium |
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| Abstract: | This study shows, by means of autoradiography, that RNA polymerase I activity is present in some of the nuclei isolated from Xenopus blastulae. This activity is localized in one or two nucleoli which have a diameter of at least 1.5 μm. The ratio between nucleolar and total nuclear grain counts allows a quantitative estimation of RNA polymerase I activity relative to total RNA polymerase activity at the blastula stage. |
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