Bleomycin resistance conferred by a drug-binding protein |
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| Authors: | A Gatignol H Durand G Tiraby |
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| Affiliation: | Laboratoire de Microbiologie et Génétique Appliquées du CNRS, CRBGC, Université Paul Sabatier, Toulouse, France. |
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| Abstract: | The protein coded by a bleomycin-resistance gene (ble) cloned from producing actinomycetes was purified from a culture of a recombinant E.coli strain and its action on bleomycin was determined by in vitro assays. The protein binds reversibly in a one to one ratio to bleomycin which can no longer cleave DNA. The bleomycin resistance of cells harboring a ble gene could be accounted for by a sequestering effect of the bleomycin-binding protein. |
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| Keywords: | Bleomycin resistance Bleomycin-binding protein DNA cleavage |
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