Immunohistochemical localization of chromogranin A and B in endocrine cells of the alimentary tract of the adult lizard Podarcis sicula |
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Authors: | Loredana D'Este Roberto Buffa Carlo Casu Nadia Carboni Micaela Pelagi Antonio G. Siccardi Tindaro Renda |
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Affiliation: | (1) Institute of Human Anatomy, University La Sapienza, Via A. Borelli 50, I-00161 Rome, Italy;(2) Third Chair of Morbid Anatomy, University of Milan, Via Commenda 9, I-20100 Milan, Italy;(3) Scientific Institute San Raffaele, I-20100 Milan, Italy |
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Abstract: | The distribution, argyrophilia, and the possible amine/peptide co-localizations in endocrine cells immunoreactive (IR) to antisera against chromogranin A (CgA) and chromogranin B (CgB) in the alimentary tract of the lizard Podarcis sicula have been investigated using novel monoclonal antibodies. Many CgA-IR and CgB-IR cells were found in the tract, except in the distal small intestine. Almost all chromogranin-IR cells (Cgs-IR) were also argyrophilic with parallel intensity. Some CgA-IR and CgB-IR cells did not display co-localized amines or peptides. CgA or CgB or both were found co-localized, with some local differences, in almost all serotonin-IR, histamine-IR, substance P-IR and gastric peptide tyrosine tyrosine (PYY)-IR cells. Moreover, both Cgs were co-localized only in some somatostatin-IR cells, whereas neurotensin-IR, gastrin/cholecystokinin-IR, pancreatic polypeptide-IR and intestinal PYY-IR cells did not show any co-localization with Cgs. The presence of Cgs in the endocrine cells was heterogeneous with regard to the complex interrelationship with their amine/peptide content. Consequently, Cgs cannot be considered as universal markers of all endocrine cell types. |
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Keywords: | Chromogranins Serotonin Histamine Regulatory peptides Gut Monoclonal antibodies Immunohistochemistry Podarcis sicula (Lacertilia) |
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