The use of high pressure liquid chromatography to study chemically induced alterations in the pattern of benzo[a]pyrene metabolism. |
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Authors: | R I Freudenthal A P Leber D Emmerling P Clarke |
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Affiliation: | Battelle Columbus Laboratories, 505 King Avenue, Columbus, Ohio 43201 U.S.A. |
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Abstract: | The metabolism of radiolabeled benzo[a]pyrene (BP) by control, 3-methyl-cholanthrene (3-MC) induced, and 1,1,1-trichloropropene-2,3-oxide (TCPO)-inhibited rat liver microsomes was measured using fluorescence, radiometric, and high-pressure liquid chromatographic (HPLC) assays. Significant differences in the total measurable metabolism of BP by the three microsomal enzyme incubations resulted from the use of the three assay procedures. Appreciable differences in the concentration of the metabolite fractions after 3-MC induction and TCPO inhibition are clearly demonstrated. NMR analysis revealed that while the 3-hydroxy-BP fraction is greater than 90% pure, the 9-hydroxy fraction contains a number of metabolites having essentially identical retention times. |
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Keywords: | BP benzo[α]pyrene HPLC high pressure liquid chromatography 3-MC 3-methyl cholanthrene NMR nuclear magnetic resonance TCPO 1,1,1-trichloropropene-2.3-oxide |
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