Long-term serial cultivation and growth requirements for human umbilical vein endothelial cells |
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Authors: | Mikio Kan Miwakato Isao Yamane |
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Affiliation: | (1) Cell Biology Division, Research Institute for Tuberculosis and Cancer, Tohoku University, 980 Sendai, Japan |
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Abstract: | Summary Human umbilical vein endothelial cells (HUV-EC) grew rapidly in vitro in medium supplemented with epidermal growth factor, fetal bovine serum (FBS) and human diploid fibroblast-conditioned medium. The effect of FBS could be replaced partially by bovine serum albumin, cholesterol, and vitamin E, and completely by further addition of serum dialysate or refeeding every other day. Among these components, fibroblast-conditioned medium is essential for HUV-EC growth. The HUV-EC were cultured serially for over 50 population doublings in the 10% FBS containing fibroblast-conditioned medium and for over 40 population doublings in the serum-free medium. Mitogenic factor(s) present in the medium conditioned by fibroblasts may be related to endothelial cell growth factor and play an important role angiogenesis and regeneration of vascular endothelium in vitro. |
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Keywords: | bovine serum albumin human umbilical vein endothelial cells human diploid fibroblasts endothelial cell growth factor |
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