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Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures
Authors:Takushi Miyoshi  Qianli Zhang  Takafumi Miyake  Shin Watanabe  Hiroe Ohnishi  Jiji Chen  Harshad D Vishwasrao  Oisorjo Chakraborty  Inna A Belyantseva  Benjamin J Perrin  Hari Shroff  Thomas B Friedman  Koichi Omori  Naoki Watanabe
Institution:1. Laboratory of Single-Molecule Cell Biology, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan;2. Department of Pharmacology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan;3. Department of Otolaryngology - Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan;4. Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA;5. Department of Nephrology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan;6. Advanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD 20892, USA;7. Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA;8. Laboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA
Abstract:
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  • Keywords:fast-dissociating antibody  espin  Fab fragment probes  single-molecule microscopy  super-resolution microscopy  light-sheet microscopy  diSPIM  TIRF microscopy  stereocilia  hair cells  F-actin turnover
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