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Integrative structure of a 10-megadalton eukaryotic pyruvate dehydrogenase complex from native cell extracts
Authors:Fotis L Kyrilis  Dmitry A Semchonok  Ioannis Skalidis  Christian Tüting  Farzad Hamdi  Francis J O’Reilly  Juri Rappsilber  Panagiotis L Kastritis
Institution:1. Interdisciplinary Research Center HALOmem, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Straße 3a, Halle/Saale, Germany;2. Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Straße 3, Halle/Saale, Germany;3. Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany;4. Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, Scotland, United Kingdom;5. Biozentrum, Martin Luther University Halle-Wittenberg, Weinbergweg 22, Halle/Saale, Germany
Abstract:
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  • Keywords:native cell extracts  cellular homogenates  keto acid dehydrogenase complexes  negative staining  cryo-EM  image processing  integrative structural biology  pyruvate dehydrogenase factory model
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