发根农杆菌的Ri-质粒转化和赛莨菪的发根培养

将赛莨菪（Scopolia Iurida)的无菌苗被含有Ri-质粒的发根农村菌（Agrobacterium rhizogenes)感染后，诱导出发根（hariy root)将发根分离，除菌后，在不含激素的琼脂或液体MS培养基上培养，发根能产生正常植物体中含有的莨菪碱和东莨菪碱等生物碱，在发根培养物中检测到agropine和mannopine，说明发根农杆菌Ri-质粒的T－DNA部分已转化到赛菪莨细胞的DNA中。

THE TRANSFERENCE OF Ri-PLASMID FROM AGROBAC-TERIUM RHIZOGENES TO SCOPOLIA LURIDA REVEALED BY THE HAIRY ROOT CULTIVATION AND THE ALKALOIDS PRODUCTION

The steriled plants of Scopolia lurida after being infected by A,rhizogenes were induced to produce hairy roots.They grew well in medium either of hormone free MS agar or of roots suspension culture.As the result,the cultivated hairy root was shown to yield alkaloids such as hyosciamine and scopolamine.Since agropine and mannopine were also detected in the hairy root cultures,the transference of the T-DNA of A.rhizogenes Ri-plasmid into the cell genomic DNA of S.lurida is clearly demonstrated.