Construction of a human ventricular cDNA library and characterization of a beta myosin heavy chain cDNA clone |
| |
| Authors: | Mark A Jandreski Choong-Chin Liew |
| |
| Institution: | (1) Department of Clinical Biochemistry, Banting Institute, University of Toronto, M5G 1L5 Toronto, Ontario, Canada;(2) Department of Medicine, Laboratory for Molecular Cardiology, Banting Institute, University of Toronto, M5G 1L5 Toronto, Ontario, Canada |
| |
| Abstract: | Summary We have constructed and characterized for the first time a complementary DNA (cDNA) clone, pHMC3, which codes for a cardiac myosin heavy chain mRNA from human heart. This clone contains a 1.7 kb DNA segment and specifies 543 amino acids of the carboxyl portion of the myosin heavy chain. The DNA sequence and encoded amino acid sequence were compared to the hamster alpha (pVHC1) and beta (pVHC2/pVHC3) cardiac myosin heavy chain cDNA and amino acid sequences and the rat cardiac myosin heavy chain sequences as well. The myosin heavy chain mRNAs are highly conserved and this is reflected in our cDNA clone. The pHMC3 clone is 87.9% homologous to the hamster alpha cDNA and 92.2% homologous to the hamster beta cDNA clones. The 3 untranslated region of pHMC3 is 64.1% homologous to the hamster beta clone while the hamster alpha myosin heavy chain shows only 25% homology to pHMC3 and exhibits extensive diversity. Similar results rere obtained when pHMC3 was compared to the rat cardiac myosin heavy chain cDNA sequences. The comparisons showed that pHMC3 is a beta cardiac myosin heavy chain cDNA clone. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
