| Construction of normal human IgE phage antibody library and the screening of the anti—trichosanthin IgE |
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| 作者姓名： | ZANHONG MINGYEH |
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| 作者单位： | [1]ShanghaiInstituteofCellBiology,ChineseAcademyofSciences,Shanghai200031,China [2]ShanghaiInstituteofCellB,ChineseAcademyofSciences,Shanghai200031,China |
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| 摘 要： | Allergen specific IgE response is the major cause of immediate hypersensitivity.However the number of IgEproducing B cells and the amount of IgE,especially the specific IgE,are so low,it greatly impedes the study of the allergic-specifc antibody responses.Here we report the construction of a normal human IgE combinatorial library.The repertoire of IgE VH genes and of κ genes were separately amplified from normal human peripheral blood lymphocytes through RT-PCR,and were then constructed to form the phage surface display human Fab(IgEVH) library.A plant protein allergen,trichosanthin(TCS),was used to affinity-enrich and to screen the anti-TCS phage HuFab clones from the library.Human IgE(Fab) to TCS were detected.
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| 关 键 词： | 健康人 IgE噬菌体抗体文库 构建 人抗TCS IgE 筛选 天花粉蛋白 |
Construction of normal human IgE phage antibody library and the screening of the anti-trichosanthin IgE |
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| ZANHONG MINGYEH.Construction of normal human IgE phage antibody library and the screening of the anti-trichosanthin IgE[J].Cell Research,1996,6(1):1-9. |
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| Authors: | ZAN HONG MING YEH |
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| Abstract: | Allergen specific IgE response is the major cause of immediate hypersensitivity. However the number of IgEproducing B cells and the amount of IgE, especially the specific IgE, are so low, it greatly impedes the study of the allergic-specifc antibody responses. Here we report the construction of a normal human IgE combinatorial library The repertoire of IgE VH genes and of K genes were separately amplified from normal human peripheral blood lymphocytes through RT-PCR, and were then constructed to form the phage surface display human Fab(IgEVH) library. A plant protein allergen, trichosanthin(TCS), was used to affinity-enrich and to screen the anti-TCS phage HuFab clones from the library. Human IgE(Fab) to TCS were detected. |
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| Keywords: | Phage antibody library human IgE(Fab) human anti-trichosanthin IgE |
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