| 染色体7q32-ter鼻咽癌相关基因的初步研究 |
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| 引用本文： | 张小慧,李忠花,张必成,董利,周鸣,曹利,唐珂,李伟芳,李桂源. 染色体7q32-ter鼻咽癌相关基因的初步研究[J]. 生物化学与生物物理进展, 2001, 28(5): 711-716 |
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| 作者姓名： | 张小慧 李忠花 张必成 董利 周鸣 曹利 唐珂 李伟芳 李桂源 |
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| 作者单位： | 中南大学湘雅医学院肿瘤研究所长沙410078 |
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| 基金项目： | 国家863项目(102-10-01-05)和973重大项目(“疾病基因组学”理论和技术体系的建立鼻咽癌子项目)资助. |
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| 摘 要： | 为克隆7q32-ter区域等位基因杂合性丢失最小共同缺失区的鼻咽癌相关基因.以STS D7S509为探针，PCR法筛选位于该区的细菌人工染色体(BAC)克隆，应用EST介导的定位-候选克隆策略并结合生物信息学筛选出在鼻咽癌细胞株和活检组织中表达增强的EST AA773454,cDNA克隆测序和生物信息学资源获取全长cDNA，DNA印迹和甲基化分析研究其表达增强的机制.结果表明，克隆的NAG18基因cDNA全长802 bp,编码227个氨基酸，定位于胞核.该基因分别与人、鼠TAXREB107基因及RPL6基因高度同源.其表达增强的机制不是基因拷贝数的丢失和甲基化位点的改变.可以断定NAG18是定位于7q32-ter最小共同缺失区的鼻咽癌相关基因，它是一高度保守的基因，参与了DNA的转录活化.
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| 关 键 词： | 7q32-ter最小共同缺失区，鼻咽癌，相关基因 |
| 收稿时间： | 2000-11-23 |
| 修稿时间： | 2000-11-23 |
Primary Study of Nasopharyngeal Carcinoma(NPC) Associated Gene on Chromosome 7q32-ter |
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| ZHANG Xiao-Hui,LI Zhong-Hu,ZHANG Bi-Cheng,DONG Li,ZHOU Ming,CAO Li,TANG Ke,LI Wei-Fang and LI Gui-Yuan. Primary Study of Nasopharyngeal Carcinoma(NPC) Associated Gene on Chromosome 7q32-ter[J]. Progress In Biochemistry and Biophysics, 2001, 28(5): 711-716 |
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| Authors: | ZHANG Xiao-Hui LI Zhong-Hu ZHANG Bi-Cheng DONG Li ZHOU Ming CAO Li TANG Ke LI Wei-Fang LI Gui-Yuan |
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| Affiliation: | Cancer Research Institute,Xiangya School of Medicine, Central South University, Changsha 410078, China;Cancer Research Institute,Xiangya School of Medicine, Central South University, Changsha 410078, China;Cancer Research Institute,Xiangya School of Medicine, Central South University, Changsha 410078, China;Cancer Research Institute,Xiangya School of Medicine, Central South University, Changsha 410078, China;Cancer Research Institute,Xiangya School of Medicine, Central South University, Changsha 410078, China;Cancer Research Institute,Xiangya School of Medicine, Central South University, Changsha 410078, China;Cancer Research Institute,Xiangya School of Medicine, Central South University, Changsha 410078, China;Cancer Research Institute,Xiangya School of Medicine, Central South University, Changsha 410078, China;Cancer Research Institute,Xiangya School of Medicine, Central South University, Changsha 410078, China |
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| Abstract: | |
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| Keywords: | nasopharyngeal carcinoma the smallest common deletion region of 7q32-ter NPC associated gene |
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