Hyperosmotic stress induces rapid synthesis of phosphatidyl-D-inositol 3,5-bisphosphate in plant cells |
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| Authors: | Harold J G Meijer Nullin Divecha Herman van den Ende Alan Musgrave Teun Munnik |
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| Institution: | (1) Institute of Molecular Cell Biology, Biocentrum, University of Amsterdam, Kruislaan 318, NL-1098 SM Amsterdam, The Netherlands, NL;(2) Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, NL-1066 CX, Amsterdam, The Netherlands, NL |
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| Abstract: | Cells from several different plant species synthesised a polyphosphoinositide (PPI)-like lipid when osmo-stressed. Synthesis
was maximal after about 10 min and was stimulated by a variety of osmolytes. Using NaCl, the strongest response centred around
200 mM. The lipid was shown to be the novel PPI isomer phosphatidyl-inositol 3,5-bisphosphate PtdIns-(3,5)P2] by analytical thin-layer chromatography and conversion to PtdIns(3,4,5)P3 using recombinant phosphoinositide 4-OH kinase. The results indicate that PtdIns-(3,5)P2 plays a role in a general osmo-signalling pathway in plants. Its potential role is discussed.
Received: 6 November 1998 / Accepted: 14 December 1998 |
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| Keywords: | :Chlamydomonas Osmotic stress Phosphatidylinositol 3 5-bisphosphate Signal transduction |
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