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Polymorphic microsatellite markers in the guava fruit fly, Bactrocera correcta (Diptera: Tephritidae) |
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| Authors: | Nopparat Buahom Yu Du Yi Wu Yuliang Deng Xiaolong Jiang Wei Fu Zhihong Li |
| Institution: | 1. Department of Entomology, College of Agriculture and Biotechnology, China Agricultural University, Yuanmingyuan West Road 2, Haidian District, Beijing, 100193, China 2. Yunnan Entry-Exit Inspection and Quarantine Bureau, No. 429, Dianchi Road, Kunming, 650228, Yunnan, China 3. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Baodaoxincun, Danzhou, 571737, Hainan, China 4. Xishuangbanna Entry-Exit Inspection and Quarantine Bureau, No. 65, Mengli Avenue, Jinghong, 666100, Xishuangbanna, Yunnan, China 5. Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Huixinli No. 241, Huixinxijie, Chaoyang District, Beijing, 100029, China |
| Abstract: | Bactrocera correcta (Bezzi) is one of the most destructive insect pests of fruits and vegetables in tropical and subtropical regions. At present, this fly is primarily distributed in Southeast Asia. Twelve microsatellite loci were isolated from an enriched genomic library based on a biotin/streptavidin capture protocol. The polymorphism of these loci was tested on 74 individual flies from two natural populations. Allele number ranged from 6 to 14 and 10 loci demonstrated a polymorphic information content (PIC) greater than 0.5. The pairwise F ST value between the two populations was 0.0048 (P > 0.05). These microsatellite loci have potential utility for studies of population genetic structure in this species. |
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