A multiplex PCR approach to simultaneously genotype potato towards the resistance alleles Ry-f
sto
and Ns |
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| Authors: | Kamil Witek Danuta Strzelczyk-?yta Jacek Hennig Waldemar Marczewski |
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| Institution: | (1) Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02106 Warsaw, Poland;(2) Plant Breeding and Acclimatization Institute, Platanowa 19, 05831 Młochów, Poland |
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| Abstract: | A simple and robust multiplex PCR approach was developed for detection of the alleles Ry-f
sto
and Ns conferring resistance of potato to Potato Virus Y (PVY) and Potato Virus S (PVS), respectively. Cleaved amplified polymorphic sequence (CAPS) markers GP122564 linked to Ry-f
sto
and SC811260 linked to Ns were amplified in one PCR reaction and identified after simultaneous digestion of the amplicons with restriction enzymes EcoRV and MboI. Effectiveness of this procedure for marker-assisted selection was confirmed in 55 potato cultivars. |
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| Keywords: | MAS Multiplex PCR Potato PVS PVY Resistance |
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