Ultrastructure of human endometrial epithelium in monolayer culture with and without steroid hormones |
| |
Authors: | Theodore M Liszczak Georges S Richardson David T MacLaughlin Paul L Kornblith |
| |
Institution: | (1) The Departments of Surgery, Gynecology, and Neurosurgery, Massachusetts General and Vincent Memorial Hospitals and Harvard Medical School, 02114 Boston, Massachusetts |
| |
Abstract: | Summary Colonies of cells of epithelioid appearance were identified in monolayer cultures grown up to 50 days from normal human endometrial
cell suspensions obtained by a method designed to insure a maximum harvest of glandular cells. Groups of these cells were
separated from stromal cells by means of cloning cylinders. Studies comparing the ultrastructure of cells of this type to
fresh endometrial tissue revealed a number of similarities. The morphological characteristics common to both types of samples
included junctional complexes, perinuclear microfilaments and microvilli with glycocalyx. Other common features were prominent
nucleoli, well developed Golgi, rough endoplasmic reticulum and membranebound electron-dense bodies in the cytoplasm. A stripping
technique applie to the fetal bovine serum used in the nutrient medium made it possible to initiate cultures in a steroidfree
environment and to maintain them in the presence of the specified concentration of estradiol and/or progesterone. Isolation
of epithelial cells of endometrium in monolayer culture may provide a useful model system in which to study the specific effects
of steroid hormones on cellular function and differentiation.
Supported by grants from the National Institutes of Health (CA 18678 and CA 07368). |
| |
Keywords: | cultures human endometrial ultrastructure estradiol progesterone |
本文献已被 SpringerLink 等数据库收录! |