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Diacylglycerol inhibits gap junctional communication in cultured epidermal cells: Evidence for a role of protein kinase C
Institution:1. German Institute of Human Nutrition Potsdam-Rehbruecke, Department of Experimental Diabetology, Potsdam-Rehbruecke, Germany;2. Division of Endocrinology, Diabetology, Angiology, Nephrology and Clinical Chemistry, Department of Internal Medicine, University Hospital Tübingen, Tübingen, Germany;3. Institute of Diabetes Research and Metabolic Diseases (IDM) of the Helmholtz Center Munich at the University of Tübingen, Tübingen, Germany;4. German Center for Diabetes Research (DZD), München-Neuherberg, Germany;1. University of Liverpool, UK;2. University of Nottingham, UK;3. University of Queensland, Australia;1. Division of Wood Chemistry, Forest Products Department, National Institute of Forest Science, 57 Hoegiro, Dongdaemun-gu, Seoul, 02455, Republic of Korea;2. Clean Energy Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea;3. School of Environmental Engineering, University of Seoul, Seoul, 02504, Republic of Korea;1. Department of Biomedical Engineering and Mechanics, Virginia Tech, 495 Old Turner Street Blacksburg, VA, 24060, 300 Norris Hall, USA;2. Department of Sociology, Virginia Tech, 225 Stanger Street, Blacksburg, VA, 24060, 562C McBryde Hall, USA;3. Department of Evolutionary Anthropology, Duke University, 130 Science Drive, Durham, NC, 27708, 203 Biological Sciences Building, USA;4. Department of Exercise Science, University of South Carolina, 921 Assembly Street Columbia, SC, 29208, United States;5. Center for Gerontology, Virginia Tech, 230 Grove Ln Blacksburg, VA, 24060, USA
Abstract:Incubation of mouse epidermal HEL-37 cells with 1-oleoyl-2-acetylglycerol (OAG) caused a dose-dependent and transient inhibition of the transfer of microinjected fluorescein between contacting cells. Soluble extracts of HEL-37 cells contained protein kinase C activity after fractionation on DEAE-cellulose and translocation of this activity to the particulate fraction occurred after 10 min exposure to OAG or to 12-O-tetradecanoylphorbol-13-acetate (TPA). After 18h exposure to TPA essentially all the protein kinase C activity was lost. In such TPA treated cells the transfer of microinjected fluorescein was refractory to inhibition by both TPA and OAG.
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