Effect of alcohols on arachidonic metabolism in murine mastocytoma cells and human polymorphonuclear leukocytes

The effects of alcohols on the formation of leukotrienes, 5-HETE prostagladin D₂ In mastocytoma cells and human neutrophils were studied. In murine mastocytoma cells, alcohols appear to have at two-different effects on the production of these arachidonic add metabolites. At low levels of cellular arachidonic acid achieved after stimulation with calcium ionophore A23187 or addition of low levels of exogenous arachidonic acid, alcohols appear to have a general inhibitory effect on the production of lipoxygenase metabolites. In the presence of higher concentrations of cellular arachidonic acid, ethanol methanol stimulated the production of lipoxygenase metabolites, but had no stimulatory effect on the cyclo-oxygenase metabolite, prostaglandin D₂. Under conditions,n-propanol t-butanol have inhibitory effects on leukotriene production. Human neutrophils are less sensitive to ethanol than mastocytoma cells, but stimulatory effects were still found at high ethanol concentrations (220–430 mM),