Effect of Formalin Fixation on the Activity of Five Enzymes of Rat Liver |
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| Authors: | Arnold M. Seligman Howard H. Chauncey Marvin M. Nachlas |
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| Affiliation: | a Yamins Laboratory for Surgical Research, Beth Israel Hospital, Bostonb Department of Surgery, Harvard Medical School, Boston |
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| Abstract: | The effect of formalin fixation on the enzymatic activity of five enzymes in rat liver was studied as a function of time and temperature, β-glucuronidase was found to be most resistant to the inactivating effects of formalin. At a time when fixation was satisfactory for preparing frozen sections (24 hours), considerable enzymatic activity was present in decreasing order; β-glucuronidase, sulfatase, acid phosphatase, esterase, and alkaline phosphatase. |
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