Antifungal Activity of Chitinases from <Emphasis Type="Italic">Trichoderma aureoviride</Emphasis> DY-59 and <Emphasis Type="Italic">Rhizopus microsporus</Emphasis> VS-9 |
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Authors: | Nam Van Nguyen Young-Ju Kim Kyung-Taek Oh Woo-Jin Jung Ro-Dong Park |
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Institution: | (1) Glucosamine Saccharide Materials, National Research Laboratory, Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju, 500-757, South Korea |
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Abstract: | Two chitinolytic fungal strains, Trichoderma aureoviride DY-59 and Rhizopus microsporus VS-9, were isolated from soil samples of Korea and Vietnam, respectively. DY-59 and VS-9 crude chitinases secreted by these
fungi in the 0.5% swollen chitin culture medium had an optimal pH of 4 and the optimal temperatures of 40°C and 60°C, respectively.
Enzymatic hydrolysis products from crab swollen chitin were N-acetyl-β-D-glucosamine (GlcNAc) by DY-59 chitinase, and GlcNAc and N, N′-diacetylchitobiose (GlcNAc)2 by VS-9 chitinases. The chitinases degraded the cell wall of Fusarium solani hyphae to produce oligosaccharides, among which GlcNAc, (GlcNAc)2, and pentamer (GlcNAc)5 were identified by high-pressure liquid chromatography. DY-59 and VS-9 chitinases inhibited F. solani microconidial germination by more than 70% and 60% at final protein concentrations of 5 and 27 μg mL−1, respectively, at 30°C for 20 h treatment. |
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