Synthesis of fluorescein labeled 7-methylguanosinemonophosphate |
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Authors: | Natarajan Amarnath Moerke Nathan Fan Yun-Hua Chen Han Christ William J Wagner Gerhard Halperin Jose A |
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Affiliation: | Laboratory for Translational Research, One Kendall Square, Building 600, 3rd Floor, Harvard Medical School, Cambridge, MA 02139, USA. |
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Abstract: | Binding of eIF4E to the cap structure (m(7)GpppN) plays a critical role in mRNA translation. To study the interaction between eIF4E and cap, and to identify small molecule inhibitors of their binding, we synthesized a fluorescent-labeled cap analogue and used it to develop a fluorescence-polarization assay. This preliminary communication describes the synthesis of a fluorescein labeled 7-methylguanosinemonophosphate, and its dose dependent binding to purified human eIF4E as demonstrated by the fluorescence polarization assay. |
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