Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway |
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Authors: | Clingenpeel Scott R Keener William K Keller Caron R De Jesus Karl Howard M Hope Watwood Mary E |
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Institution: | Department of Biological Sciences, Idaho State University, Pocatello, ID 83209, USA. |
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Abstract: | 3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate. |
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