Inhibition of Water Melon (Citrullus vulgaris) Urease by Fluoride |
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Authors: | Om Prakash Lata Shea Bachan Upadhyay |
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Affiliation: | 1. Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221 005, India
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Abstract: | Effect of fluoride on the activity of purified urease from seeds of watermelon (Citrullus vulgaris) was studied. Fluoride exhibited a concentration dependent inhibition both in presenceand absence ofthe substrate. The inhibition was non-competitive. Addition of 8mM β-mercaptoethanol gradually abolished the fluoride inhibition. β-mnercaptoethanol, in presence of fluoride, also exhibited a concentration dependent suppression of inhibition caused by fluoride. The significance of these observations is discussed. |
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