Membrane glycoproteins of Chlamydomonas eugametos flagella |
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Authors: | Alan Musgrave Wieger Homan Wies van den Briel Nico Lelie Dick Schol Loes Ero Herman van den Ende |
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Institution: | (1) Plantenfysiologisch Laboratorium, Universiteit van Amsterdam, Ijdijk 26, Amsterdam, The Netherlands |
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Abstract: | The flagellar glycoproteins exposed on Chlamydomonas eugametos gametes were labeled by means of lactoperoxidase, diiodosulfanilic acid and chloramine T, and characterised in SDS-electrophoresis gels. The medium from gamete cultures contains particles (isoagglutinins) that agglutinate gametes of the opposite mating type. When crude preparations of these particles were subjected to isopycnic centrifugation in a caesium chloride gradient, two bands of particles were found. The lighter, active band consisted of membrane vesicles. The denser, inactive band consisted of cell wall material. The active band had the same glycoprotein composition as membrane vesicles artificially made from isolated flagella. Preparations of glagella were also separated on a caesium chloride cushion into pure flagella and cell wall material. The flagella, but not the cell wall material, isoagglutinated opposite gametes. Again the glycoprotein composition of pure flagella was similar to that of pure isoagglutinin vesicles. No difference was detected between the protein and glycoprotein compositions of flagella and isoagglutinins from both mating types.Abbreviations LPO
lactoperoxidase
- PB
phosphate buffer
- DISA
diazotized 125I-iodo-sulfanilic acid
- SDS
sodium dodecyl sulphate
- CBD
coomassie Brilliant Blue
- PAS
periodic acid Schiff |
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Keywords: | Chlamydomonas Flagella Glyco-proteins Membrane |
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