Germination of concentrated suspensions of spores from Aspergillus niger |
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Authors: | J Barrios-González C Martínez A Aguilera M Raimbault |
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Institution: | (1) Departmento de Biotecnología, Universidad Autónoma Metropolitana, Apatado Postal 55-535, 09340 Mexico, D.F. |
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Abstract: | Summary Germination of condiospores fromA.niger in very concentrated suspension was required to inoculate solid fermentation media, but a germination self-inhibitor was detected in spores. It was found that the inhibitory effect depended on the composition of the medium and was reduced when glucose was used as a carbon source. The effect of the self-inhibitor was eliminated by washing the spores and using glucose and a protein nitrogen source in the germination medium. By this method it was possible to increase about 100 times (106 to 108) spore concentration, keeping more than 90% germination. |
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