Effects of laser radiation on rhus vernicifera laccase,type 2 Cu-depleted laccase,and stellacyanin |
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| Authors: | Giovanni Musci Lucia Tosi Alessandro Desideri Laura Morpurgo Arlette Garnier-Suillerot |
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| Affiliation: | Département de Recherches Physiques, Universite Pierre et Marie Curie, Paris, France;Dipartimento di Fisica, Università di Calabria, Arcavacata, Italy;Centro di Biologia Moleculare del C.N.R., Istituto di Chimica Biologica, Università di Roma Italy;Département de Recherches Physiques, Université Pierre et Marie Curie and Laboratoire de Chimie Bioinorganique, U.E.R. de Sante, Medecine et Biolog, Paris, France |
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| Abstract: | Laser irradiation in the 450 nm region brings about irreversible changes in the copper sites of Rhus vernicifera laccase and its type 2 Cu-depleted derivative. The absorption band at 614 nm disappears after ~ 2 hr of irradiation with a 200 mW laser beam; the amount of the paramagnetic detectable copper does not decrease, indicating no reduction of these types of copper. No apparent rearrangement of the protein backbone occurs, as ultaviolet dichroic spectra of the enzyme before and after the irradiation do not show appreciable differences. Stellacyanin is insensitive to laser radiation at any wavelength. |
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| Keywords: | Address reprint requests to Dr. Lucia Tosi Département de Recherohes Physiques Université Pierre and Marie Curie L.A. 71 4 place Jussieu 752 |
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