Characterization of Esterases in a Brazilian Population of Zaprionus Indianus (Diptera: Drosophilidae) |
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| Authors: | L G C Galego C R Ceron C M A Carareto |
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| Institution: | 1. Departamento de Biologia, UNESP – Universidade Estadual Paulista, Rua Cristóv?o Colombo, 2265, 15054-000, S?o José do Rio Preto, SP, Brazil
2. Departamento de Química e Ciências Ambientais, UNESP – Universidade Estadual Paulista, Rua Cristóv?o Colombo, 2265, 15054-000, S?o José do Rio Preto, SP, Brazil
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| Abstract: | The aim of this study was to characterize esterases in Zaprionus indianus, a drosophilid recently introduced into Brazil. A further aim was study the variation of activity of esterases in the presence
of inhibitors and their expression according to sex, sexual activity and age of individual flies. Polymorphisms were detected
in two esterase loci (Est-2 and Est-3) and monomorphisms in four others (Est-1, Est-4, Est-5 and Est-6). Biochemical tests
using α- and β-naphthyl acetate and the inhibitors malathion, eserine sulphate and PMSF allowed us to classify EST-2 and EST-5 as β-esterases, both carboxyl-esterases, and EST-1, EST-3, EST-4 and EST-6 as α-esterases. EST-1 and EST-3 were classified as carboxyl-esterases and EST-4 and EST-6 as cholinesterases. EST-5 activity was
more pronounced in males and EST-2 was restricted to them or to recently copulated females. EST-4, rarely detected, was not
characterized. Based on their biochemical characteristics possible roles for these enzymes are suggested. |
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| Keywords: | β -esterases α -esterases carboxyl-esterases cholinesterases esterases Zaprionus indianus |
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