Microwave-enhanced enzyme reaction for protein mapping by mass spectrometry: a new approach to protein digestion in minutes |
| |
Authors: | Pramanik Birendra N Mirza Urooj A Ing Yao Hain Liu Yan-Hui Bartner Peter L Weber Patricia C Bose Ajay K |
| |
Affiliation: | Schering-Plough Research Institute, Kenilworth, New Jersey 07033, USA. birendra.pramanik@spcorp.com |
| |
Abstract: | Accelerated proteolytic cleavage of proteins under controlled microwave irradiation has been achieved. Selective peptide fragmentation by endoproteases trypsin or lysine C led to smaller peptides that were analyzed by matrix-assisted laser desorption ionization (MALDI) or liquid chromatography-electrospray ionization (LC-ESI) techniques. The efficacy of this technique for protein mapping was demonstrated by the mass spectral analyses of the peptide fragmentation of several biologically active proteins, including cytochrome c, ubiquitin, lysozyme, myoglobin, and interferon alpha-2b. Most important, using this novel approach digestion of proteins occurs in minutes, in contrast to the hours required by conventional methods. |
| |
Keywords: | Microwave irradiation accelerated protein digestion mass spectrometry selective endoprotease reactions |
本文献已被 PubMed 等数据库收录! |
|