RAPD and FAME analyses of Astragalus species growing in eastern Anatolia region of Turkey |
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| Affiliation: | 1. Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR, USA;2. Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA;1. National Autonomous University of Nicaragua, León, Nicaragua;2. School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA;3. Department of Epidemiology, University of North Carolina at Chapel Hill Gillings School of Global Public Health, USA;4. Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA;5. National Virology Laboratory, Centro Nacional de Diagnóstico y Referencia, Ministry of Health, Managua, Nicaragua;6. Division of Molecular Virology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden |
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| Abstract: | Fatty acid (FAs) and RAPD profiles were used to examine phenotypic and genetic relationships between eight Astragalus species including Astragalus maximus Willd. var. maximus, Astragalus coadunatus Hub. Mor. & Chamb., Astragalus kurdicus Boiss. var. kurdicus, Astragalus lagurus Willd, Astragalus christianus L., Astragalus cicer L., Astragalus atrocarpus Champ & Matthews and Astragalus onobrychioides Bieb., which were wildly growing in eastern Anatolia region of Turkey. All of the eight Astragalus species tested in this study were separated based on the presence and composition of 45 different FAs. Four of the Astragalus species including A. coadunatus, A. lagurus, A. christianus, and A. atrocarpus were rich in terms of FA contents containing at least 22–31 different FAs. The relative proportions of two fatty acids, 16:0, and 18:1:ω8c were higher in these four Astragalus species. The remaining species have limited number of FAs with unique FAMEs profiles. Six of the 10 decamer primers examined were selected to find out genetic polymorphism in Astragalus species. A total of 98 polymorphic bands were observed, ranging in size from 250 bp to 3000 bp. The RAPD results suggested that A. atrocarpus, A. onobrychioides and A. kurdicus are closely related and completely different from the other species. Six genetically distinct groups were found among the species of Astragalus. High genetic variations among Astragalus species growing wildly in eastern Anatolia region of Turkey may imply the differences in their origins. The results in the present study suggested that both RAPD and FA analyses are useful for differentiation of Astragalus species. |
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