Application of 2D BN/SDS-PAGE coupled with mass spectrometry for identification of VDAC-associated protein complexes related to mitochondrial binding sites for type I brain hexokinase |
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| Authors: | Carla Rossini Crepaldi Phelipe Augusto Mariano Vitale Andrea Cristina Tesch Hélen Julie Laure José César Rosa Marcelo de Cerqueira César |
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| Affiliation: | 1. Department of Basic Sciences, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil;2. Protein Chemistry Center and Department of Molecular and Cellular Biology and Pathogenic Bioagents, Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil |
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| Abstract: | Two types of binding sites for hexokinase, designated as Type A or Type B sites, have been shown to coexist on brain mitochondria. The ratio of these sites varies between species.HK1 attaches by reversibly binding to the voltage dependent anion channel (VDAC). Regarding the nature of hexokinase binding sites, we investigated if it was linked to distinct VDAC interactomes. We approached this question by 2D BN/SDS-PAGE of mitochondria, followed by mass spectrometry.Our results are consistent with the possibility that the ratio of Type A/Type B sites is due to differential VDAC interactions in bovine and rat neuronal cells. |
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