dRecQ4 Is Required for DNA Synthesis and Essential for Cell Proliferation in Drosophila
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| Authors: | Yanjuan Xu Zhiyong Lei Hai Huang Wen Dui Xuehong Liang Jun Ma Renjie Jiao |
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| Institution: | 1. State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, the Chinese Academy of Sciences, Beijing, China.; 2. Graduate School of the Chinese Academy of Sciences, Beijing, China.; 3. Divisions of Biomedical Informatics and Developmental Biology, Cincinnati Children''s Hospital Research Foundation, Cincinnati, Ohio, United States of America.;University of Minnesota, United States of America |
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| Abstract: | BackgroundThe family of RecQ DNA helicases plays an important role in the maintenance of genomic integrity. Mutations in three of the five known RecQ family members in humans, BLM, WRN and RecQ4, lead to disorders that are characterized by predisposition to cancer and premature aging.Methodology/Principal FindingsTo address the in vivo functions of Drosophila RecQ4 (dRecQ4), we generated mutant alleles of dRecQ4 using the targeted gene knock-out technique. Our data show that dRecQ4 mutants are homozygous lethal with defects in DNA replication, cell cycle progression and cell proliferation. Two sets of experiments suggest that dRecQ4 also plays a role in DNA double strand break repair. First, mutant animals exhibit sensitivity to gamma irradiation. Second, the efficiency of DsRed reconstitution via single strand annealing repair is significantly reduced in the dRecQ4 mutant animals. Rescue experiments further show that both the N-terminal domain and the helicase domain are essential to dRecQ4 function in vivo. The N-terminal domain is sufficient for the DNA repair function of dRecQ4.Conclusions/SignificanceTogether, our results show that dRecQ4 is an essential gene that plays an important role in not only DNA replication but also DNA repair and cell cycle progression in vivo. |
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