Molecular cloning and expression in Escherichia coli of bleomycin-resistance gene from a methicillin-resistant Staphylococcus aureus and its association with IS431 mec |
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Authors: | M Z A Bhuiyan K Ueda Y Inouye M Sugiyama |
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Institution: | (1) Department of Paediatrics, Hiroshima University School of Medicine, Kasumi 1-2-3, 734 Minami-ku, Hiroshima, Japan;(2) Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Kasumi 1-2-3, 734 Minami-ku, Hiroshima, Japan |
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Abstract: | A gene that confers bleomycin resistance was cloned from the chromosomal DNA of methicillin-resistant Staphylococcus aureus (MRSA) B-26 into the plasmid anC18. It is of chromosomal origin rather than plasmid and exists in the chromosome making a cluster with the kanamycin-resistance gene. We found that the nucleotide sequence of the bleomycin-resistance gene from the chromosome of MRSA B-26 is identical to that from a staphylococcal plasmid, pUB110. The partial sequence of IS431mec was also found upstream from the DNA fragment containing the bleomycin- and kanamycin-resistance genes. |
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