Aminopeptidase N is a marker for the apical pole of porcine thyroid epithelial cells in vivo and in culture |
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Authors: | Hélène Feracci Alain Bernadac Sonia Hovsépian Guy Fayet Dr Suzanne Maroux |
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Institution: | (1) CNRS-CBM, Marseille;(2) Faculté de Médecine, Laboratoire de Biochimie Médicale et U38 INSERM, Marseille, France;(3) C.N.R.S.-C.B.M., 31, Chemin Joseph Aiguier, P.O. Box 71, F-13277 Marseille Cedex 9, France |
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Abstract: | Summary An aminopeptidase N has been detected by immunofluorescence in the apical plasma membrane of porcine thyroid cells, facing the follicular lumen. Freshly isolated cells obtained by tissue trypsinization, lose their polarity and exhibit a homogeneous enzyme distribution over the whole plasma membrane. In thyrotropin-stimulated cultured cells organized into follicles, the enzyme is localized in the apical cell pole. In monolayer cells, on the other hand, the enzyme is distributed over the whole surface facing the medium. In both types of cultures fluorescence is also observed in intracytoplasmic organelles. In vivo, aminopeptidase is a marker of the apical part of the thyroid plasma membrane, but its in vitro localization depends upon cell differentiation related to the culture conditions. |
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Keywords: | Aminopeptidase Thyroid Thyroid cell Membrane marker Polarization |
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