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Somaclonal variation of haploid in vitro tissue culture obtained from Siberian larch (Larix sibirica Ledeb.) megagametophytes for whole genome de novo sequencing |
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| Authors: | Konstantin V Krutovsky Iraida N Tretyakova Nataliay V Oreshkova Maria E Pak Olga V Kvitko Eugene A Vaganov |
| Institution: | 1. Department of Forest Genetics and Forest Tree Breeding, Büsgen-Institute, Georg-August-University of G?ttingen, Büsgenweg 2, 37077, G?ttingen, Germany 2. Department of Ecosystem Science and Management, Texas A&M University, College Station, TX, 77843-2138, USA 3. N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkina, Moscow, 119333, Russia 4. Genome Research and Education Center, Siberian Federal University, 50a/2 Akademgorodok, Krasnoyarsk, 660036, Russia 5. Laboratory of Forest Genetics and Selection, V. N. Sukachev Institute of Forest, Siberian Branch of Russian Academy of Sciences, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russia 6. Department of Ecology and Environmental Studies, Institute of Economics, Management and Environmental Studies, Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, 660041, Russia |
| Abstract: | The objective of this study was to obtain a genetically stable haploid in vitro-derived line from Siberian larch (Larix sibirica Ledeb.) using megagametophyte explants, which then could be used for different molecular genetic studies, including whole genome de novo sequencing. However, cytogenetic analysis and genotyping of 11 microsatellite loci showed high levels of genomic instability and a high frequency of mutation in the obtained megagametophyte-derived callus cultures. All cultures contained new mutations in one or more microsatellite loci. |
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