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Supramolecular-mediated immobilization of l-phenylalanine dehydrogenase on cyclodextrin-coated Au electrodes for biosensor applications |
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| Authors: | Reynaldo Villalonga Akira Fujii Hiroaki Shinohara Yasuhisa Asano Roberto Cao Shinjiro Tachibana Pedro Ortiz |
| Institution: | (1) Center for Enzyme Technology, University of Matanzas, Autopista a Varadero Km. 3 1/2, Matanzas, CP 44740, Cuba;(2) Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan;(3) Field of Life, Information and System Sciences, Graduate School of Science and Engineering for Research, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan;(4) Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan;(5) Laboratory of Bioinorganic Chemistry, Faculty of Chemistry, Havana University, Havana, 10400, Cuba |
| Abstract: | A supramolecular approach was used for adsorbing a monolayer of adamantane-modified phenylalanine dehydrogenase on β-cyclodextrin-coated Au electrodes. The enzyme electrode (poised at +200 mV vs. Ag/AgCl) showed a linear amperometric response up to 3 mM l-phenylalanine (l-Phe) with a lower detection limit of 15 μM. The reversible nature of this immobilization approach was confirmed. |
| Keywords: | Biosensor β -Cyclodextrin Enzyme immobilization Phenylalanine dehydrogenase Supramolecular complex |
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