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Effects of culture conditions on molecular weights of poly(3-hydroxyalkanoates) produced byPseudomonas putida from octanoate
Authors:Katsutoshi Hori  Kazuo Soga  Yoshiharu Doi
Institution:(1) Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, 227 Yokohama, Japan;(2) Polymer Chemistry Laboratory, The Institute of Physical Chemical Research (RIKEN), Hirosawa 2-1, Wako-shi, 351-01 Saitama, Japan
Abstract:Summary Time-dependent changes in the molecular weights of poly(3-hydroxyalkanoates) (P(3HA)) produced byPseudomonas putida from octanoate were investigated. The mole ratio of carbon to nitrogen sources (C/N), incubation temperature, concentration of octanoate, and pH of culture solution were varied to study the effects of culture conditions on the yield and molecular weights of P(3HA). The molecular weights of P(3HA) decreased with time to reach a constant value during incubation. When the incubation temperature and the concentration of octanoate were low, P(3HA) polymers of high molecular weights were produced.
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