| PEG介导的外源基因对绿豆叶肉原生质体的转化和瞬间表达研究(简报) |
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| 引用本文： | 黄健秋,卫志明,许智宏.PEG介导的外源基因对绿豆叶肉原生质体的转化和瞬间表达研究(简报)[J].分子细胞生物学报,1991(4). |
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| 作者姓名： | 黄健秋 卫志明 许智宏 |
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| 作者单位： | 中科院上海植物生理研究所 植物分子遗传国家重点实验室
(黄健秋，卫志明)，中科院上海植物生理研究所 植物分子遗传国家重点实验室(许智宏) |
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| 摘 要： | 作者曾利用Krens等(1982)的PEG法将NPT-Ⅱ丛因直接导入绿豆原生质体并获得稳定表达,但该法的转化率极低。为了建立绿豆原生质体有效的转化系统,我们将PEG法在大豆原生质体转化的基础上进一步改进,利用GUS基因为报告基因,对三个不同品种的绿豆叶肉原生质体进行直接转化。并对影响PEG转化的有关因素进行了研究。
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| 关 键 词： | 绿豆 原生质体 瞬间表达 |
STUDIES ON TRANSIENT EXPRESSION OF FOREIGN GENE IN MESOPHYLL PROTOPLASTS OF PHASEOLUS AUREUS |
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| Huang Jian-qiu,Wei Zhi-ming and Xu Zhi-hong.STUDIES ON TRANSIENT EXPRESSION OF FOREIGN GENE IN MESOPHYLL PROTOPLASTS OF PHASEOLUS AUREUS[J].Journal of Molecular Cell Biology,1991(4). |
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| Authors: | Huang Jian-qiu Wei Zhi-ming and Xu Zhi-hong |
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| Abstract: | The E. Coli beta-glucuronidase (GUS) gene was used in transient expression experiments with mesophyll protoplasts of Phaseolus aureus. Transient expression of GUS gene could be detected 44 hours after DNA incorporation into protoplasts by using a flurogenic substrate MUG. The results indicated that transformation efficiency was increased by increasing plasmid DNA concentration, carrier DNA was not beneficial when transforming with circular plasmid DNA, and PEG 40% (13.3% in final) was more suitable than PEG 24% (8% in final) when other affecting factors were identical. |
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| Keywords: | Phaseolus aureus Protoplast Transient Expression |
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