Freeze-drying of solutions of serum albumin containing dimethylsulfoxide. |
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Authors: | D Greiff B T Doumas T I Malinin B W Perry |
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Institution: | 1. Department of Pathology, The Medical College of Wisconsin, Milwaukee, Wisconsin 53233, USA;2. Department of Surgery, University of Miami School of Medicine, Miami, Florida 33152 USA |
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Abstract: | Although several publications have emphasized the inadvisability of drying biologic materials containing dimethylsulfoxide (DMSO) by sublimation of ice in vacuo, our studies showed a relationship to exist between the relative concentrations of serum albumin, human, and dimethylsulfoxide for successful or unsuccessful freeze-drying of albumin-DMSO solutions. The cycle of freeze-drying for the successful drying of an albumin-DMSO solution was a modification of the cycle used for the successful drying of suspensions of measles virus by sublimation of ice in vacuo. Using nuclear magnetic resonance spectroscopy, a strong, sharp signal for DMSO was obtained in preparations of freeze-dried albumin-DMSO solutions rehydrated with deuterium oxide, D2O. |
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