Adipose Tissue Dysfunction Occurs Independently of Obesity in Adipocyte‐Specific Oncostatin Receptor Knockout Mice |
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| Authors: | Jacqueline M. Stephens Jennifer L. Bailey Hardy Hang Victoria Rittell Marilyn A. Dietrich Randall L. Mynatt Carrie M. Elks |
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| Affiliation: | 1. Adipocyte Biology Laboratory, Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA;2. Matrix Biology Laboratory, Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA;3. Cell Biology and Bioimaging Core, Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA;4. Transgenics Core, Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA |
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| Abstract: | Objective This study examined the phenotypic effects of adipocyte‐specific oncostatin M receptor (OSMR) loss in chow‐fed mice. Methods Chow‐fed adipocyte‐specific OSMR knockout (FKO) mice and littermate OSMRfl/fl controls were studied. Tissue weights, insulin sensitivity, adipokine production, and stromal cell immunophenotypes were assessed in epididymal fat (eWAT); serum adipokine production was also assessed. In vitro, adipocytes were treated with oncostatin M, and adipokine gene expression was assessed. Results Body weights, fasting blood glucose levels, and eWAT weights did not differ between genotypes. However, the eWAT of OSMRFKO mice was modestly less responsive to insulin stimulation than that of OSMRfl/fl mice. Notably, significant increases in adipokines, including C‐reactive protein, lipocalin 2, intercellular adhesion molecule‐1, and insulinlike growth factor binding protein 6, were observed in the eWAT of OSMRFKO mice. In addition, significant increases in fetuin A and intercellular adhesion molecule‐1 were detected in OSMRFKO serum. Flow cytometry revealed a significant increase in leukocyte number and modest, but not statistically significant, increases in B cells and T cells in the eWAT of OSMRFKO mice. Conclusions The chow‐fed OSMRFKO mice exhibited adipose tissue dysfunction and increased proinflammatory adipokine production. These results suggest that intact adipocyte oncostatin M–OSMR signaling is necessary for adipose tissue immune cell homeostasis. |
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