Characterisation,screening and selection of Bacillus subtilis isolates for its biocontrol efficiency against major rice diseases |
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| Authors: | M. K. Prasanna Kumar Narayanappa Amruta C. P. Manjula M. E. Puneeth Kalavathi Teli |
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| Affiliation: | 1. Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, India;2. Department of Seed Science and Technology, University of Agricultural Sciences, Bengaluru, India |
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| Abstract: | A Soil bacterium, Bacillus subtilis, isolated from the rhizosphere of rice, showed high biocontrol activity against blast, sheath blight and bacterial blight. In the present study, four B. subtilis strains isolated from paddy soil were studied under laboratory, greenhouse and field conditions. Among the four strains assayed, UASP17 gave maximum inhibition of paddy pathogens and was validated under field trials. B. subtilis (UASP17) under different doses and methods of applications was evaluated for two seasons at Agriculture Research Station (UAS, Raichur). UASP17 was effective in reducing the severity of blast (9.00% and 15.57%), bacterial leaf blight (BLB) (5.00% and 6.11%) and sheath blight (11.93% and 4.17%) diseases for two seasons. The application of bioagent also increased the paddy grain yield (61.00 and 64.30?Q/ha) in the two seasons, respectively. Taken together, these results indicate that B. subtilis UASP17 as seedling dip for 30?min (10?mL/L of water) prior to transplanting and 2.50?L/ha foliar application was effective in managing the diseases of paddy. |
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| Keywords: | B. subtilis strains rice diseases bioassay biocontrol |
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