Cloning,expression, and purification of an antiviral protein from Pseudomonas fluorescens CZ and its antagonistic activity against tobacco mosaic virus |
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| Authors: | Lili Shen Fangfang Li Yubing Jiao Hangjun Sun |
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| Affiliation: | 1. Plant Protection College, Shenyang Agricultural University, Shenyang, China;2. Tobacco Research Institute, Chinese Academy of Agricultural Science, Qingdao, China;3. Tobacco Research Institute, Chinese Academy of Agricultural Science, Qingdao, China |
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| Abstract: | The antiviral alkaline phosphatase L (AapL) protein of Pseudomonas fluorescens CZ was expressed in Escherichia coli, purified using the Ni-NTA column, and its antiviral activity against tobacco mosaic virus (TMV)-infected Nicotiana was tested. The recombinant protein (360?µg/mL) showed stability and most effective suppression in vitro (94.85%) against TMV. |
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| Keywords: | Pseudomonas fluorescens antiviral protein expression and purification antagonistic activity |
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