Influence of DNA isolation on Q-PCR-based quantification of methanogenic Archaea in biogas fermenters |
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| Authors: | I. Bergmann K. Mundt M. Sontag I. Baumstark E. Nettmann M. Klocke |
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| Affiliation: | 1. Leibniz-Institut für Agrartechnik Potsdam-Bornim e.V., Abteilung Bioverfahrenstechnik, Max-Eyth-Allee 100, D-14469 Potsdam, Germany;2. Technische Universität Berlin, Institut für Technischen Umweltschutz, Fachgebiet Umweltmikrobiologie, Franklinstrasse 29, D-10587 Berlin, Germany |
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| Abstract: | Quantitative real-time PCR (Q-PCR) is commonly applied for the detection of certain microorganisms in environmental samples. However, some environments, like biomass-degrading biogas fermenters, are enriched with PCR-interfering substances. To study the impact of the DNA extraction protocol on the results of Q-PCR-based analysis of the methane-producing archaeal community in biogas fermenters, nine different protocols with varying cell disruption and DNA purification approaches were tested. |
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| Keywords: | Biogas Continuously stirred tank reactor DNA extraction protocol Methanogenic archaea Microbial community structure Quantitative real-time PCR |
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