槲皮素对甲硫氨酸负载大鼠氨基酸代谢的影响

为探讨槲皮素对甲硫氨酸(Met)负载大鼠氨基酸代谢的影响,将Wistar大鼠24只随机分为3组,即对照组、1％甲硫氨酸组以及1％甲硫氨酸和0.5％槲皮素组,喂养6周后,采用高压液相色谱法测定血清中半胱氨酸含量,全自动氨基酸分析仪测定血清中其他氨基酸含量.结果显示,1％甲硫氨酸干预后除对牛磺酸产生显著影响外,对其他氨基酸没有明显影响.0.5％槲皮素干预后,血清必需氨基酸苏氨酸、缬氨酸含量较1％ Met组显著升高(p＜0.05),牛磺酸、缬氨酸、亮氨酸和异亮氨酸较对照组亦显著升高(p＜0.05),而血清丝氨酸和脯氨酸含量较对照组显著降低(p＜0.05).结果表明,槲皮素可能加强甲硫氨酸转硫化代谢途径.

Effect of Quercetin on Regulation of Amino Acid Metabolism in Rat Induced by High Methionine Diet

To investigate the effects of quercetin on amino acid metabolism,twenty four male Wistar rats were randomly divided into three groups and fed for 6weeks.Group 1was the control.Group 2was fed with 1% methionine.Group 3was fed with 1% methionine and 0.5% quercetin.Concentrations of amino acid in serum were assayed by high performance liquid chromatography and amino acid analyzer respectively.Compared with group of1% methionine,threonine and valine were decreased significantly in 0.5% quercetin group(p<0.05).Compared with the control,taurine,valine,leucine and isoleucine were increased significantly,but serine and proline were decreased significantly in 0.5% quercetin group(p<0.05).The results suggested that quercetin probably increased methionine transsulfuration metabolism.