Comparative analysis of nuclear proteins of B cells in different developmental stages |
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Authors: | Jin Sun Hee Cho Eun Hye Ko Jung Eun Jung Eun Hee Ahn Byungchan Hahm Jong Ryeal Kim Jae Won Kim Choong Won Kim Deok Ryong |
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Affiliation: | Department of Biochemistry, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju 660-751, South Korea. |
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Abstract: | The developmental stage-specific regulation of V(D)J recombination, a gene rearrangement process of antigen receptor gene segments, is tightly controlled in cells. Here we screened proteins uniquely or differentially expressed among three developmentally distinguishable B cells (pro-B, pre-B and mature B cells) using two-dimensional gel electrophoresis and mass spectrometry. Chromatin assembly factor 1 was uniquely expressed in pro-B cells. Purine nucleotide phosphorylase, LCK, E2A and many other unidentified proteins were dominantly present in the nucleus at the early stage of B cell development where the V(D)J recombination process occurs. Also, few proteins including guanidine nucleotide binding proteins were exclusively expressed in pre-B cell. Such findings can provide some information to help understand the developmental regulation of gene rearrangement occurring during B cell development. |
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