Nearby clusters of hemagglutinin residues sustain SLAM-dependent canine distemper virus entry in peripheral blood mononuclear cells |
| |
Authors: | von Messling Veronika Oezguen Numan Zheng Qi Vongpunsawad Sompong Braun Werner Cattaneo Roberto |
| |
Institution: | Molecular Medicine Program, Mayo Clinic, Guggenheim 1838, 200 1st Street SW, Rochester, MN 55905, USA. |
| |
Abstract: | Signaling lymphocytic activation molecule (SLAM, CD150) is the universal morbillivirus receptor. Based on the identification of measles virus (MV) hemagglutinin (H) amino acids supporting human SLAM-dependent cell entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient canine SLAM-dependent membrane fusion. These residues are located in two nearby clusters in a new CDV H structural model. To completely abolish SLAM-dependent fusion, combinations of mutations were necessary. We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret peripheral blood mononuclear cells while retaining full infectivity in epithelial cells. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|