|
|||||
|
|
Cell wall active antibiotics reduce chromosomal DNA fragmentation by peptidoglycan hydrolysis in Staphylococcus aureus |
|
| Authors: | María Tamayo Rebeca Santiso Jaime Gosálvez Germán Bou María del Cármen Fernández José Luis Fernández |
| Institution: | 1. Genetics Unit, INIBIC-Complejo Hospitalario Universitario A Coru?a (CHUAC), As Xubias, 84, 15006, A Coru?a, Spain 2. Molecular Genetics and Radiobiology Laboratory, Centro Oncológico de Galicia, 15009, A Coru?a, Spain 3. Genetics Unit, Facultad de Biología, Universidad Autónoma de Madrid, 28049, Madrid, Spain 4. Microbiology Division, INIBIC-Complejo Hospitalario Universitario A Coru?a (CHUAC), 15006, A Coru?a, Spain |
| Abstract: | Lysostaphin digestion of peptidoglycan (PG) from Staphylococcus aureus resulted in chromosomal DNA fragmentation by released DNase, as directly visualized in situ on isolated nucleoids. Nevertheless, DNA digestion was partially prevented by previous incubation with antibiotics that inhibit PG synthesis. This inhibitory effect was much more remarkable with glycopeptides vancomycin and mainly teicoplanin than with beta-lactams cloxacillin and ceftazidime. Therefore, inhibition of PG chain elongation has a more significant inhibition of DNA degradation than inhibition of PG cross-linking, possibly due to a reduction in DNase storage at the cell wall. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |