Synthesis and molecular recognition of carbohydrate-centered multivalent glycoclusters by a plant lectin RCA120 |
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| Authors: | Gao Yongjun Eguchi Atsuko Kakehi Kazuaki Lee Yuan C |
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| Institution: | Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA. |
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| Abstract: | Water soluble and lectin-recognizable carbohydrate-centered glycoclusters were prepared efficiently by the Huisgen 1,3-cycloaddition reaction of methyl-2,3,4,6-tetra-O-propargyl beta-D-galactopyranoside with 2-azidoethyl glycosides of lactose and N-acetyllactosamine. Their binding by a plant lectin RCA120 was examined by capillary affinity electrophoresis using fluorescence-labeled asialoglycans from human alpha1-acid glycoprotein. The glycoclusters showed 400-fold stronger inhibitory effect than free lactose, manifesting strong multivalency effect. |
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